어스바이오는 Cytelligen 한국 공식 대리점입니다.
어스바이오는 Cytelligen 한국 공식 대리점으로서 모든 제품을 전문적으로 취급 및 공급하고 있습니다.
[Cytelligen 한국공식대리점] CTC & CTEC Technology
Aneuploid Circulating Tumor Cell (CTC) and Circulating Tumor-derived Endothelial Cell (CTEC)
Tumor-derived endothelial cells (TECs)는 종양 미세환경 내 신생 혈관의 내벽을 구성합니다(Hida and Klagsbrun, 2005 Cancer Res 65:2507). 말초 혈액으로 유출된 후, Tumor cells (TCs)와 TECs는 각각 Circulating TCs (CTCs)와 Circulating TECs (CTECs)로 전환됩니다. TCs/CTCs와 TECs/CTECs는 모두 이수성 염색체의 세포유전학적 이상을 지닙니다(Lin et al., 2017 Sci Rep 7:9789). 순환 종양 바이오마커의 독특한 범주를 구성하는 이수성 CTCs와 CTECs는 순환 과정에서 상호작용하며 종양 발생, 신생혈관 형성, 질병 진행 및 암 전이를 촉진합니다. 저산소성 종양 미세환경에서 일부 CD31- 종양 세포는 CD31+ 종양 유래 내피 세포로 전분화될 수 있습니다(Lin, 2020 Cells 9:1539).
Subtraction Enrichment (SE) to Enrich Circulating Rare Cells
Principle
- Non-hemolytic removal of RBCs via Cytelligen’s non-hematologic cell separation matrix
- Depletion of WBCs (4-5 logs) by highly specific immuno-magnetic particles conjugated to a unique cocktail of anti-leukocytes monoclonal antibodies
- Independent of target cell surface molecules (such as EpCAM) and cell size. Enrich non-hematologic circulating rare cells including CTCs and CTECs in large or small cell size (≤ 5 μm WBCs)
- Source of specimens: patients (any volume of blood, routine 6 ml) or mice (50-500 μl of blood, routine 200 μl). In addition to blood, specimens can also be collected from bone marrow, pleural effusion, ascites, cerebrospinal fluids, urine, punctured lymph node, cervical smear, etc.
- Enriched, viable circulating rare cells are suitable for in vitro primary tumor cell culture and various downstream analyses
i•FISH® to Identify Diverse Subtypes of CTCs and CTECs
Principle
- Cytelligen's patented technology: integrating immunostaining of multiple tumor markers and fluorescence in situ hybridization (FISH) to perform in situ phenotypic and karyotypic characterization of aneuploid CTCs and CTECs
- SE-i•FISH® encompasses every aspect of the cellular tri-elements including nucleic acids, protein expression and cell morphology
- Time-saving
- Up to six colors: available for self-selection of any tumor markers expressed on the plasma membrane, in the cytoplasm or the nucleus
i•FISH® to Comprehensively Co-detect and Characterize Diverse Subtypes of CTCs and CTECs
Both aneuploid CTCs and CTECs can be classified into diverse subtypes upon degree of aneuploidy, tumor marker expression and cell morphology. Each subtype possesses a distinct clinical significance, such as tumor progression, therapy resistance and cancer relapse, etc... iFISH-identified CTCs and CTECs are available for subsequent NGS-based single cell analyses.
SE-i•FISH® (NC) effectively identifies and comprehensively co-detects viable and necrotic CTCs、CTECs
SE-i•FISH®(NC) is able to effectively enrich, detect, characterize and subcategorize various necrotic and viable CTCs and CTECs. Each subtype of CTCs and CTECs possesses distinct utility in anti-cancer drug development, translational research and clinical practice regarding risk stratification, rapid evaluation of therapeutic effects, real-time monitoring of treatment-resistance, and detection of post-therapeutic minimal residual disease (MRD).
어스바이오(USBIO)는 Cytelligen 한국 공식 대리점입니다.
해당 제품에 대한 문의나 Cytelligen 제품의 견적 또는 문의사항이 있으시면 아래로 연락주시기 바랍니다.
Tel: 02-862-2816 / Email: bio@usbio.co.kr
